Biomedika <p style="text-align: justify;">JURNAL<strong> BIOMEDIKA</strong> is a peer-reviewed open access journal covers basic and advance research in biomedical science, with focus on the biomedical laboratory science and technology research. The journal is published by the Faculty of Health Sciences, Setia Budi University to promote scientific writing and dissemination of knowledge in biomedical laboratory science and technology. Scientific community are encouraged to submit original research articles and reviews with topicsincluding but not limited to clinical chemistry, hematology, cytohistology, immunoserology, microbiology, toxicology, molecular biology, biochemistry, chemical biology, and food analysis. J. Biomedika is open for submission all year round, and published semiannually.&nbsp;This journal also has become a CrossRef Member&nbsp;since March 2019. Therefore, all articles published by this journal will have unique DOI number.&nbsp;<strong>JURNAL BIOMEDIKA&nbsp;</strong>(<em>p-ISSN:</em><em>1979-035X</em><em>, e-ISSN:&nbsp;</em><em>2302-1306</em>) has been published since 2008. Research articles published in the early 2008 that are not listed on this website have been stored on this link&nbsp;<strong></strong></p> <p style="text-align: justify;"><strong>Editorial Office :</strong><br><strong>Faculty of Health Sciences&nbsp;<br>Setia Budi University, Surakarta, Indonesia&nbsp;</strong></p> Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta en-US Biomedika 1979-035X The Effect of Variations of Red Bricks, Sand, Sawdust, and Green Tea (Camellia sinensis) Media on the Fat Content of Salted Egg Yolks <p>Salting is done to extend the shelf life of duck eggs. A variety of media can be used for salting eggs, including a mixture of salt, sand, red brick, sawdust, and brewed tea. Salted eggs are popular among the general public, but they contain a lot of fat, which, if consumed in excess, can cause blood vessel blockage (atherosclerosis). Green tea is a plant that has many benefits, one of which is that it can help people lose weight. Duck eggs were ripened for 12 days in a variety of salting media with different ingredients for this study. Red bricks, sand, sawdust, salt, and brewed green tea are among the materials used. This study aimed to determine the fat content of salted egg yolks using the Soxhlet extraction method, as well as the color, aroma (smell), and taste of salted eggs. The data in this study were analyzed using One-Way ANOVA and the results revealed that variations in salting media had a significant effect on fat content in salted egg yolks. The results showed the changes in fat content in salted egg yolks without treatment with a fat content of 36.28 percent, and with treatment with media A (sand, red bricks, and sawdust); media B (sand, red bricks, and sawdust); media 1 (media A added with green tea); and media 2 (media B added with green tea), with the fat contents of 35.22%; 32.86%, and 30.49%. &nbsp;Mixed media of green tea affects the fat content of salted egg yolks, from 36.50% to 32.86% and 30.49%.</p> Nur Hidayati Mardiyono Mardiyono Dian Kresnadipayana ##submission.copyrightStatement## 2022-09-21 2022-09-21 15 1 1 7 10.31001/biomedika.v15i1.1203 Determination Levels of Caffeine Isolated from Robusta Coffee Beans from Pagar Alam in Wistar Rat Blood <p>Coffee provides a genuine source of caffeine. Caffeine can have a variety of pharmacological effects on both central and peripheral nervous systems and most of these effects are related to the antagonistic mechanism at adenosine receptors. The most prominent effect of caffeine remains stimulation of brain function, improving mood and physical performance. Robusta coffee obtains an intense level of caffeine and Pagar Alam City is one of the cities in Indonesia that produces robusta coffees. This study aims to identify and characterize caffeine isolated from Pagar Alam robusta coffee beans using thin-layer chromatography methods, UV-Visible spectrophotometry and to determine caffeine levels in wistar rat blood using the ELISA method. This method is cost-effective, familiar to perform and has a considerable degree of accuracy and reproducibility. The isolated samples have similar characteristics to standard caffeine with an Rf value of 0.77, a maximum wavelength at 273 nm with the absorbance of 1.3 and from caffeine profile in the blood sample we obtained the maximum sample concentration in blood 0.582 ± 0.249 μg/ml and the time needed to reach the maximum concentration is 30 minutes.</p> Yunida Yunida Muhammad Totong Kamaluddin Theodorus Theodorus Sonlimar Simangunsong ##submission.copyrightStatement## 2022-09-22 2022-09-22 15 1 8 15 10.31001/biomedika.v15i1.1251 A Hepatoprotective Effect of Extract Ethanol Gynura procumbens on Liver Injury Induced by Toxic Dose of Paracetamol <p>Gynura procumbens is a plant that contains flavonoid compounds as antioxidants and has a hepatoprotective effect. A hepatoprotector is a compound that can protect the liver from liver damage. One way to determine liver function is to measure the enzyme activity of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT). This study aimed to determine the hepatoprotective effect of the extract ethanol of Gynura procumbens on the liver induced by paracetamol toxic dose. This study used 20 white male rats divided into five groups: positive control group, negative control (NaCMC 1%), and paracetamol dose of 2.400mg/kg bw). EDSNY 100 mg/kg bw, 200 mg/kg bw, 300 mg/kg bw + 2.400 mg/kg paracetamolThe rat blood samples were taken through the lateral vein, and then the SGOT and SGPT were measured before, after 4 days, and on the 6th day after paracetamol induction. The results showed that the ethanol extract of Gynura procumbens significantly reduced the levels of SGPT in mice at a dose of 300 mg/kg bw. However, the ethanol extract of Gynura procumbens leaves could not reduce SGOT levels in rats. From the histopathological results, it was evident that both low, medium, and high doses had not been able to significantly improve liver damage induced by toxic doses of paracetamol. However, the ethanol extract of Gynura procumbens leaves appeared to reduce the number of areas of necrosis and degeneration of hepatocytes compared to the negative control group. Based on the study's results, the ethanol extract of Gynura procumbens leaves at a dose of 300 mg/kg bw was able to protect against an increase in SGPT. Still, it hadn't shown that it was the best way to protect the liver from damage caused by too much paracetamol.</p> Sri Almarahma Peter Kabo Yulia Yusrini Djabir ##submission.copyrightStatement## 2022-09-23 2022-09-23 15 1 16 24 10.31001/biomedika.v15i1.1289 Comparison Results of Hemoglobin Checkup in Pregnant Women using Cyanmethemoglobin Method and Azidemet Hb Methods <p>The hemoglobin checkup in pregnant women was done by several methods, some of those were Cyanmethemoglobin and Azidemet Hb methods. The incidence of anemia during pregnancy, childbirth and during the puerperium can be reduced by early detection of anemia by checking hemoglobin levels. The purpose of this study was to determine hemoglobin levels using the Cyanmethemoglobin method and the Azidemet Hb method. Data collection was carried out by observational method using cross sectional design. The research was conducted at the Ajibarang II Public Health Center, Banyumas Regency in October - November 2020. The sample used was 20 respondents. The results of the examination using the Cyanmethemoglobin method for venous blood were 10.12 ± 0.62 g / dL and the Azidemet Hb method for capillary blood was 11.98 ± 1,0009 g/dL. There is a significant difference between the results of hemoglobin examination using the Cyanmethemoglobin method of venous blood and the Azidemet Hb method of capilary blood.</p> Arif Mulyanto Fitri Paramitha Annisa Tantri Analisawati Sudarsono ##submission.copyrightStatement## 2022-09-26 2022-09-26 15 1 25 30 10.31001/biomedika.v15i1.1204 Apple Juice to Reduce Total Cholesterol Level in Women Over 40 Years Old <p>Cholesterol is a fatty substance in the human body’s cells. A person who has high cholesterol levels may develop coronary heart disease (CHD), which increases their risk of developing atherosclerosis. Due to pregnancy, hormonal, and menopausal reasons, women over the age of 40 are more likely to have elevated cholesterol levels than younger women. Apples' fiber content has been shown to reduce blood cholesterol levels. This study aimed to determine the effect of apple juice on cholesterol levels.&nbsp; A pretest-posttest group design with a pre-experimental methodology was used in this study. 38 women between the ages of 40 and 66 made up the&nbsp;sample of this study. Every morning for seven days, respondents received apple juice. The study was carried out in August 2020. According to this study, apple juice has a positive effect in lowering total cholesterol levels. Prior to receiving apple juice, the mean total cholesterol level was 226.68 17.230 mg/dL, while the mean cholesterol level after apple juice treatment was 184.39 13.771 mg/dL. Total cholesterol levels before and after receiving apple juice are known to differ by 42.29 mg/dL. The Paired T-Test analysis of the data revealed a value of P = 0.000 (P 0.05), indicating that there was an effect of giving apple juice on lowering respondents' total cholesterol levels.</p> Muallima Tulummah Retno Sulistiyowati Kurnia Ritma Dhanti ##submission.copyrightStatement## 2022-09-26 2022-09-26 15 1 31 35 10.31001/biomedika.v15i1.1185