Biomedika <p style="text-align: justify;">JURNAL<strong> BIOMEDIKA</strong> is a peer-reviewed open access journal covers basic and advance research in biomedical science, with focus on the biomedical laboratory science and technology research. The journal is published by the Faculty of Health Sciences, Setia Budi University to promote scientific writing and dissemination of knowledge in biomedical laboratory science and technology. Scientific community are encouraged to submit original research articles and reviews with topicsincluding but not limited to clinical chemistry, hematology, cytohistology, immunoserology, microbiology, toxicology, molecular biology, biochemistry, chemical biology, and food analysis. J. Biomedika is open for submission all year round, and published semiannually.&nbsp;This journal also has become a CrossRef Member&nbsp;since March 2019. Therefore, all articles published by this journal will have unique DOI number.&nbsp;<strong>JURNAL BIOMEDIKA&nbsp;</strong>(<em>p-ISSN:</em><em>1979-035X</em><em>, e-ISSN:&nbsp;</em><em>2302-1306</em>) has been published since 2008. Research articles published in the early 2008 that are not listed on this website have been stored on this link&nbsp;<strong></strong></p> <p style="text-align: justify;"><strong>Editorial Office :</strong><br><strong>Faculty of Health Sciences&nbsp;<br>Setia Budi University, Surakarta, Indonesia&nbsp;</strong></p> en-US (Dian Kresnadipayana) (Rumaeyda Citra Puspita) Thu, 30 Sep 2021 00:00:00 +0000 OJS 60 Performance Test of Homemade Control Materials from Addition and Spike Placebo Simulation on Urine Chloride Examination with Fantus Method <p>The background of this research was to examine the performance of homemade urine control materials on urine chloride examination. This study was aimed to test the performance of homemade control materials from addition and spike placebo simulation on urine chloride examination using the Fantus method. This research applied an experimental research method. The stages of the study started with standardization of AgNO<sub>3</sub> solution, preparation of urine control materials from addition and spike placebo simulation, as well as calculation of accuracy (%R), bias (%d) and coefficient of variation (%CV). The results showed that the normal and pathological levels of accuracy in the urine control materials from addition were 109.55% and 104.30%, while the accuracy values (%R) ​​for urine control materials from spike placebo simulation, for the normal and pathological levels, were high, 110.07% and 104.54%.&nbsp; The bias values (%d) obtained in the urine control materials from addition with normal and high pathological levels were 9.55% and 4.30% bias, while the bias values (%d) in the urine control materials from spike placebo simulation with normal and high pathological levels were 10.07% and 4.54%. The coefficient variation (%CV) values ​​in urine control materials from addition at normal and pathological high levels were 3.75% and 3.07%, while the %CV values ​​in urine control materials from spike placebo simulation at normal and high pathological levels were 4.55% and 3.11%. This study concludes that based on the accuracy (%R) and bias (%d) parameters, the homemade control materials from addition and spike placebo simulation have relatively good performance for urine chloride examination using the Fantus method. Meanwhile, based on the parameter of coefficient of variation (%CVV), the homemade control materials from the addition and spike placebo simulation have slightly lower performance for examining urine chloride with the Fantus method.</p> Titin Aryani ##submission.copyrightStatement## Wed, 01 Sep 2021 00:00:00 +0000 The Comparison of Mycobacterium tuberculosis Detection using Molecular Rapid Test and Immunochromatography in Patients Suspected of Having Tuberculosis in Pangkajene and Kepulauan Regencies <p><em>Mycobacterium tuberculosis</em> is an acid-resistant bacterium that causes tuberculosis and can be detected using a variety of methods. This study aimed to determine the comparison of the detection results of <em>Mycobacterium tuberculosis</em> using the molecular rapid test (MRT) and immunochromatographic method. This research was conducted at Batara Siang Hospital, Pangkajene, and Kepulauan Regencies, in July-September 2019 using 100 samples. This study is cross-sectional research, applying <em>the chi-square</em> test for analysis. The results of statistical tests revealed a significant difference between the results of tests using molecular rapid tests and immunochromatography (&lt;0.001). The sensitivity, specificity, positive probability value, negative probability value, and accuracy of the immunochromatography method against the molecular rapid test (MRT) were 91.3%, 100%, 100%, 93.1%, and 96%, respectively.</p> Faradillah Suhar, Muhammad Nasrum Massi, Rizalinda Sjahril ##submission.copyrightStatement## Sat, 18 Sep 2021 00:00:00 +0000 Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components <p>Concentrated platelet components are the components or platelets that are processed through centrifugation from whole blood or a single donor. The components of the concentrate platelets are stored in an agitator at a temperature of 20-24◦C with a maximum shelf-life of five days. During the storage, a change in component metabolism occurs, which is characterized by alteration in platelet morphology as an indicator of decreasing quality of platelet components. The purpose of this study was to determine the quality of the components of platelets based on the shelf-life by measuring the platelet morphological values with the Kunicki method. This study applied an experimental method by conducting inspections of the regional blood transfusion unit (UTDD) of the Indonesian Red Cross of&nbsp; Jakarta Capital Special Region (PMI DKI Jakarta). Platelet morphological values of six components of platelets were examined by the Kunicki method on day 0 to day 5 of shelf-life. Quality assessment was carried out based on a significant difference-dependent t-test and comparison with normal value. The results of the study by performing statistical analysis of the significant difference-dependent t-test showed a significant difference of values between day 0 and day 1 to day 5 of shelf-life. Comparison with normal values ​​depicted that all samples stored on day 4 had a morphological value of more than 200. On day 5 of shelf-life, the platelet morphology values ​​of five samples were below 200. The quality of the concentrated platelet components is said “good” if it is above 200. This study concluded that there was a significant difference in the platelet morphology values between the components of concentrated platelets on day 0 and day 1 to day 5 of shelf-life. All samples were of good quality until day 4 of storage. However, on day 5 of storage, only one sample was good in quality.</p> Dewi Astuti, Eva Ayu Maharani, Hafshoh Susadma ##submission.copyrightStatement## Sun, 26 Dec 2021 00:00:00 +0000 The Analysis of Vitamin D Receptor Protein on Salmonella typhi infection in acute recurrent cases in endemic area in Eastern Indonesia <p>The host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank&nbsp; Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with <em>Enzyme-linked Immunosorbent Assay</em> (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression.</p> Fatmawati Annisa Syamsuddin, Mochammad Hatta, Firdaus Hamid, Rosdiana Natzir, Ahyar Ahmad, Burhanuddin Bahar ##submission.copyrightStatement## Fri, 31 Dec 2021 00:00:00 +0000 Subacute Toxicity Test of (Scaevola taccada Gaertn.) Roxb Leaf Extract on Kidney and Liver Function in Rats (Rattus norvegicus) <p>Beruwas laut leaf (<em>Scaevola taccada</em> (Gaertn.) Roxb has pharmacological effects such as anti-inflammatory, analgesic, antioxidant, antidiabetic and anticancer. Although beruwas laut leaf have many advantages, safety is the main requirement that herbal medicine must have. This research was aimed to prove sebacute of beruwas laut leaf (<em>Scaevola taccada</em> (Gaertn.) Roxb.) about the function of kidney and liver. This research used 20 rats were devided into 4 groups. Group 1 as a control group and groups 2,3, and 4 as an experimental group by administration of beruwas laut leaf extract with dose 200 mg/kgBB, 400 mg/kgBB, and 600 mg/kgBB. The extract was made using maceration and sebacute toxicity testing was carried out for 14 days. After giving the extract, some of rats had diarrhea. The results showed significant effect to increase levels of ureum and CGT after administration of dose 400mg/kgBB. Moreover, administration of dose 600 mg/kgBB caused significant effect in liver biomarkers and kidney (GGT and ureum). It was concluded that ethanol extract of beruwas laut leaf (Scaevola taccada (Gaertn.) Roxb.) with dose 200mg/kgBB showed safe but toxic to kidney and liver with dose 600mg/kgBB<em>.</em></p> Tenri Ayu Adri, Aritzah Dwi Widati, Peter Kabo, Yulia Yusrini Djabir ##submission.copyrightStatement## Tue, 28 Dec 2021 00:00:00 +0000