Antibacterial Activity of Ethanol Extract, n-Hexana, Ethyl Acetate And Water Fraction From Wangon Leaf (Olax scandens Roxb.) Against Staphylococcus aureus ATCC 25923

Abstract

Wangon leaves contained chemical compounds such as saponins, tannins, flavonoids,

steroids and phenols. The aim of this study was to determine antibacterial activity of ethanol

extract, n-hexane, ethyl acetate, and water fraction from wangon leaves to Staphylococcus

aureus ATCC 25923 bacteria.

Wangon leaves powder was extracted by maceration method with solvent ethanol

96%. Wangon leaf extract that has been obtained then was fractionated using n-hexane, ethyl

acetate and water as solvent. Ethanol extract and fractions were tested for its antibacterial

activity using a diffusion test with 50%, 25%, and 12.5% concentration to find out which

fraction was the most effective. The most effective fraction was tested in dilutions method

with various concentrations ie, 50%; 25%; 12.5%; 6.25%; 3.125%; 1.562%; 0.781%;

0.391%; 0.195%; 0.098% to determine the minimum inhibitory concentration and minimum

kill concentration.

The results showed that the ethanol extract, n-hexane, ethyl acetate, and water

fraction had antibacterial activity against Staphylococcus aureus ATCC 25923. The 50%

concentration of ethyl acetate fraction was the most effective against Staphylococcus aureus

ATCC 25923 and had an inhibition zone diameter of 12.5 mm. But the Minimum Inhibitory

Concentration was unknown and Minimum Kill Concentration was 25%.