Antioxidant Potential of Ipomoea carnea Jacq Leaves Extract Fractions Based on Solvent Optimization and Soxhletation Time Using the DPPH Method

  • Nova Rahma Widyaningrum STIKES Mamba'ul 'Ulum SUrakarta
  • Yesi Ihdina Fityatal Hasanah Universitas Islam Negeri Raden Mas Said Surakarta
  • Mella Santika Azhari Pharmacy, STIKES Mamba'ul 'Ulum Surakarta
  • Sitta Hasanattin Sholehah Universitas Muhammadiyah AR Fachruddin
DOI: https://doi.org/10.31001/jfi.v23i01.3092

Abstract

The content of Ipomoea carnea Jacq leaves includes flavonoids, saponins, phenolics and alkaloids, which can be used as anti-inflammatory, antioxidant, antidiabetic, antimicrobial, wound healing, immunomodulator, cardiovascular treatment, embryotoxic and antifungal. The purpose of this study was to examine the antioxidant potential derived from the optimal fraction of the extraction solvent and soxhletation time using the DPPH method. Extraction of the contents of krangkungan leaves was carried out with methanol solvent for 5 hours, then fractionation was carried out using n-hexane, ethyl acetate and methanol solvents and then tested for potential antioxidant activity using the DPPH method. Activity measurements were carried out using a UV-VIS spectrophotometer with a wavelength of 526.4nm. The antioxidant results showed that all fractions had antioxidant activity, but had different categories. The IC50 values ​​of n-hexane were 115.125 ppm (moderate), ethyl acetate 82.804 ppm (strong), and methanol 51.63 ppm (strong) respectively, with a routine comparator of 24.23 ppm (very strong). The results of statistical analysis using the ANOVA test showed significant differences between the three fractions and the routine comparator.

Keywords: Antioxidant Ipomoea carnea Jacq Methanol fraction Soxhletation DPPH methode

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Published
2026-05-31
Issue
Vol 23 No 01 (2026): JURNAL FARMASI INDONESIA
Section
Articles