Antiasthma Activity Of Combination Of Decoction Of Sembung Leaves (Blumea balsamifera L. DC.), Patikan Kebo Herbs (Euphorbia hirta L.), Kemukus Seeds (Piper cubeba L.), and Teki Rhizomes (Cyperus rotundus Linn.) at Male Guinea Pig In Vitro and In Vivo

Abstrak

Empirically, combination of decoction of sembung leaves, patikan kebo herbs, kemukus fruits, and teki rhizomes has been used to relief asthma. Individually, those plants known had activity as antiasthma. The goal of the research are to assess the activity of combination of decoction in different proportion as antiasthma in vitro and in vivo.
The antiasthma activity testing in vitro is done using isolated guinea pig traceal muscle in the Krebs Buffer without calcium. CaCl2 used as inductor of trachea smooth muscle contraction. Percent of contraction before and after immersion of decoction compared to reveal the activity of decoction as a smooth muscle contraction inhibitor. The antiasthma activity in vitro testing is done using guinea pig as asthma animal model. Asthma inductor was subcutan and nebulation of ovalbumin. Asthma guinea pig were given combination of decoction for 29 days. Tracheal smooth muscle stained with hematoxilin-eosin to count eosinophils, and percent of edema.
The result showed that one kind of decoction has activity as contraction inhibitor of smooth muscle trachea at minute 14 – 26. There was no different eosinophil count and percent of smooth muscle edema. The decoction has no activity as eosinophil infiltration inhibitor and edema of smooth muscle trachea