ANTIBACTERIAL ACTIVITY TEST OF KEPEL LEAF EXTRACT FRACTION (Stelechocarpus burahol, Hook F&Th.) AGAINST THE BACTERIUM Propionibacterium acnes AND DETECTION OF ACTIVE FRACTION CONTENT
Abstract
Kepel leaf (Stelechocarpus burahol, Hook F&Th.) has antibacterial activity. The objective was to determine the most active antibacterial fraction of kepel leaf extract against Propionibacterium acnes bacteria, determine the value of inhibitor diameter and KBM and detect the content of active fractions. The method of diffusion with disc discs. Dilution method and Thin-layer Chromatography. The results showed that the most active water fraction, tested by the diffusion method, obtained a resistance diameter of 21.53±0.66. The water fraction tested by the dilution method had a KBM value of 2%. The water fraction contains alkaloid compounds and tannins.
Keywords:
Antibacterial Propionibacterium acnes Kepel leaf Extraction Fractionation
References
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[2] Putra, A. (2020). Profil Penderita Acne Vulgaris Yang Mendapatkan Terapi Antibiotik Oral Dan Topikal Di Balai Kesehatan Kulit, Kelamin Dan Kosmetika Makassar Periode 2018-2019. In Energy for Sustainable Development: Demand, Supply, Conversion and Management (Issue November).
[3] McLaughlin, J., Watterson, S., Layton, A. M., Bjourson, A. J., Barnard, E., & McDowell, A. (2019). Propionibacterium acnes and acne vulgaris: New insights from the integration of population genetic, multi-omic, biochemical and host-microbe studies. Microorganisms, 7(5). https://doi.org/10.3390/microorganisms7050128
[4] Sundari, D., Handayani, D. S., & Suryanti, V. (2023). Identifikasi senyawa dari ekstrak metanol daging buah kepel (Stelechocarpus burahol). Pros Sem Nas Masy Biodiv Indon, 9(1), 86–90. https://doi.org/10.13057/psnmbi/m090113
[5] Hidayah, W. W., Kusrini, D., & Fachriyah, E. (2016). Isolasi, Identifikasi Senyawa Steroid dari Daun Getih-Getihan (Rivina humilis L.) dan Uji Aktivitas sebagai Antibakteri. Jurnal Kimia Sains Dan Aplikasi, 19(1), 32. https://doi.org/10.14710/jksa.19.1.32-37
[6] Aries, T., Susanti, C., Azis, A., Rasyid, R. A., Weno, I., & Tahamata, Y. (2022). Kandungan Kualitatif Senyawa Metabolisme Sekunder Ekstrak Etanol Daun Pandemor Asal Pulau Biak. Jurnal Kehutanan Papuasia, 8(1), 47–54
[7] Arsyad, R., Amin, A., & Waris, R. (2023). Teknik Pembuatan Dan Nilai Rendamen Simplisia Dan Ekstrak Etanol Biji Bagore (Caesalpinia Crista L.) Asal Polewali Mandar. Makassar Natural Product Journal, 1(3), 2023–2138. https://journal.farmasi.umi.ac.id/index.php/mnpj
[8] Waras Nurcholis, Fachrur Rizal Mahendra, Milanda Fiorella Gultom, Safira Khoirunnisa, Mayang Anggita Cahya Kurnia, & Hamdan Hafizh Harahap. (2022). Phytochemical, Antioxidant and Antibacterial Screening of Orthosiphon stamineus Leaf Extract Two Phenotypes. Jurnal Jamu Indonesia, 7(3), 121–129. https://doi.org/10.29244/jji.v7i3.280
[9] Siswanto, B. D. (2014). Uji daya anti bakteri dan efikasi larutan kumur ekstrak buah kepel Stelechocarpus burahol dalam mengontrol Halitosis Fisiologis = Antibacterial test and effication of extract Kepel Fruits (Stelechocarpus Burahol) mouthwash in controlling halitosis fisiolo. Fakultas Farmasi Universitas Indonesia, sumber day. https://lib.ui.ac.id/detail?id=20420153&lokasi=lokal
[10] Tumiar Pakpahan, D. (2020). Antibacterial Activity Of N-Hexane, Ethyl Acetate, And Butanol Fraction Of Lead Tree (Leucaena Leucocephala (Lam.) De Wit) Leaves Against Propionibacterium Acnes And Staphylococcus epidermidis. Indonesia Natural Research Pharmaceutical Journal, 5(2), 12–19.
[11] Suzan Astyamalia, Maulana Tegar Aditya Nugraha, & Ferli Eko. (2023). Uji Aktivitas Antibakteri Ekstrak Etanol Kombinasi Daun Kepel (Stelechocarpus Burahol) dan Pegagan (Centella Asiatica). INSOLOGI: Jurnal Sains Dan Teknologi, 2(2), 378–384. https://doi.org/10.55123/insologi.v2i2.1832
[12] Lawal, M. M., L., & S. A, A. (2019). Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from GuieraSenegalensis (Leaves). Open Access Journal of Chemistry, 3(3), 7–12. https://doi.org/10.22259/2637-5834.0303002
[13] Marlina, S. D., Suryanti, V., & Suyono, S. (2005). The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.). Biofarmasi Journal of Natural Product Biochemistry, 3(1), 26–31. https://doi.org/10.13057/biofar/f030106
[14] Kemenkes. (2017). Formularies. Pills and the Public Purse, 236–238. https://doi.org/10.2307/jj.2430657.12
[15] Wijayati, N., Astutiningsih, C., & Mulyati, S. (2014). Transformasi α-pinena dengan bakteri Pseudomonas aeruginosa ATCC 25923. Biosaintifika, Journal of Biology & Biology Education, 6(1), 24–28.
[16] Ramadani, A., Rahayu, Y. P., Nasution, M. P., & Yuniarti, R. (2023). Analisis cemaran bakteri Staphylococcus aureus pada daging ayam krispy pinggir jalan dan fast food di daerah Teladan kota Medan. Journal of Pharmaceutical and Sciences, 6(3), 1265–1272. https://doi.org/10.36490/journal-jps.com.v6i3.205
[17] Koirewoa, Y. A., Fatimawali, & Wiyono, W. I. (2012). Isolation And Identification Flavonoid Compounds In Beluntas Leaf (Pluchea Indica L .). Jurnal Farmasi, 47–52.
[18] Kurniasih, H. (2019). Uji Aktivitas Tabir Surya Ekstrak Daun Kepel (Stelechocarpus Burahol [Blume] Hook F. & Th.) Secara In Vitro.
[19] Fahrunnida, Rarastoeti Pratiwi. (2015). Kandungan Saponin Buah , Daun dan Tangkai Daun Belimbing Wuluh ( Averrhoa bilimbi L .) The Content of Saponin in Fruits , Leaves and Petioles of Belimbing Wuluh ( Averrhoa bilimbi L .). Seminar Nasional Konservasi Dan Pemanfaatan Sumber Daya Alam 2015, Universitas Gajah Mada, Fakulttas Biologi, 220–224.
[20] Yuda, P. E. S. K., Cahyaningsih, E., & Winariyanthi, N. P. Y. (2017). Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Tanaman Patikan Kebo (Euphorbia hirta L.). Jurnal Ilmiah Medicamento, 3(2), 61–70. https://doi.org/10.36733/medicamento.v3i2.891
[21] Jayani, N. I. E., & Handojo, H. O. (2021). Standarisasi Simplisia Daun Tempuyung (Sonchi Folium) Hasil Budidaya Di Ubaya Training Center Trawas Mojokerto. Journal of Pharmacy Science and Technology, 1(1), 68–79. https://doi.org/10.30649/pst.v1i1.59
[22] Sutomo, S., Hasanah, N., Arnida, A., & Sriyono, A. (2021). Standardisasi Simplisia dan Ekstrak Daun Matoa (Pometia pinnata J.R Forst & G. Forst) Asal Kalimantan Selatan. Jurnal Pharmascience, 8(1), 101. https://doi.org/10.20527/jps.v8i1.10275
[23] Shadrina, A. N., Widyanengsih, E., Eiko, N. B., Putri, N. A., Sulastri, N., Dzulfiana, N., Rajebi, O., & Sulvita, W. (2022). Analisis Fitokimia Dan Aktivitas Farmakologi Tanaman Kepel (Stelechocarpus Burahol) Terhadap Beberapa Penyakit : Review. Jurnal Buana Farma, 2(3), 14–21. https://doi.org/10.36805/jbf.v2i3.546
[24] Nuria, M. C., Chabibah, Z., Banu, S., & Fithria, R. F. (2016). Ekstrak Metanol Daun Gugur Ketapang ( Terminalia catappa L .) Sebagai Antidiare. Jurnal Ilmu Farmasi Dan Farmasi Klinik, 1(1), 163–173.
[25] Ujianti, R. M. D., & Androva, A. (2019). Analysis of Water Quality and River Waters Microbology for Manifestation of Food Safety. Advance Sustainable Science, Engineering and Technology, 1(1), 1–7. https://doi.org/10.26877/asset.v1i1.4877
Published
2025-07-23
How to Cite
PRIATEN, E. (2025). ANTIBACTERIAL ACTIVITY TEST OF KEPEL LEAF EXTRACT FRACTION (Stelechocarpus burahol, Hook F&Th.) AGAINST THE BACTERIUM Propionibacterium acnes AND DETECTION OF ACTIVE FRACTION CONTENT. Journal of Pharmaceutical Researchers, 3(2). https://doi.org/https://doi.org/10.31001/jpr.v2i2.2686
Section
Article
