Efficacy Test of Kopasanda Leaf Extract Ointment (Chromolaena odorata L) in Accelerating Burn Wound Healing
Abstract
Burns are skin injuries that can cause significant damage, increase the risk of diseases, and negatively impact mental health and quality of life. One plant traditionally used to treat burns is Chromolaena odorata (kopasanda). Kopasanda leaves are known for their various benefits, including anti-inflammatory properties and their ability to support wound healing. This study aims to evaluate ointments containing kopasanda leaf extract formulated at three concentrations: 10%, 15%, and 20%. The objectives of the study are to determine whether ointments with concentrations of 10%, 15%, and 20% meet good physical quality standards and to test their effectiveness in accelerating burn wound healing in male white rabbits.
The extraction of kopasanda leaves was carried out using the maceration method with 96% ethanol, and the ointment was formulated using a polyethylene glycol hase. The test animals, male white rabhits, were inflicted with burns on their back areas, and the healing process was observed from the first day to the 21st day. Data analysis was performed using one-way ANOVA and the Least Significant Difference test.
The results showed that the kopasanda leaf extract ointment met good physical quality standards, and the 20% concentration was the most effective in accelerating the healing second-degree burns. This effectiveness is supported by the active compounds in kopasanda leaves, such as flavonoids, tannins, and saponins.
References
[1] G. R. W. Sanjaya, N. M. Linawati, I. G. K. N. Arijana, I. A. I. Wahyuniari, and I. G. N. S. Wiryawan, “Flavonoid dalam penyembuhan luka bakar pada kulit,” J. Sains Kes., vol. 5, no. 2, pp. 243–249, Jun. 2023, doi: 10.25026/jsk.v5i2.1247.
[2] Y. A. Babo and O. K. Putri, “Uji parameter spesifik ekstrak etanol 70% daun kopasanda (Chromolaena odorata L.),” J. Ilm. Farm. Indones., pp. 1–7, 2018.
[3] N. Z. Sabri and H. Yusof, “Chromolaena odorata: A review of its antimicrobial activity and its application in medicine,” Bul. Sains Kes., vol. 5, no. 2, pp. 1–9, Oct. 2021.
[4] M. Zahara, “Deskripsi Chromolaena odorata L.R.M. King dan H. Robinson sebagai tanaman obat: Suatu tinjauan,” in IOP Conf. Ser.: Mater. Sci. Eng., vol. 506, no. 1, p. 012022, Apr. 2019, doi: 10.1088/1757-899X/506/1/012022.
[5] R. Triyandi, M. Iqbal, M. F. Wardhana, D. A. Ramdini, V. F. Puteri, A. F. A. Palogan, A. Fredison, and M. Kanedi, “Burns healing rate in rats medicated with leaf extract of tekelan (Chromolaena odorata) ointment,” World J. Pharm. Pharm. Sci., vol. 9, no. 12, pp. 178–186, 2021.
[6] R. Helmidanora, H. Wijaya, and Musakir, “Potential Activity of Serunai Leaf Ethanol Extract (Chromolaena Odorata L.) Against the Healing of Burns,” Iraqi J. Pharm. Sci., vol. 32, no. 2, pp. 91–95, 2023, doi: 10.31351/vol32iss2pp91-95.
[7] V. S. Rosa, F. Hasanah, and D. N. Siahaan, “Testing of the effects of balakacida (Chromolaena odorata L.) leaf extract ointment on wound healing in guinea pigs,” J. Farmasimed (JFM), vol. 5, no. 2, pp. 148–156, 2023.
[8] Kementerian Kesehatan Republik Indonesia, Farmakope Herbal Indonesia, ed. 2. Jakarta: Kemenkes RI, 2017.
[9] M. Nurhajanah, L. Agussalim, S. Z. Iman, dan T. L. Hajiriah, “Analisis kandungan antiseptik daun kopasanda (Chromolaena odorata) sebagai dasar pembuatan gel pada luka,” Bioscientist: Jurnal Ilmiah Biologi, vol. 8, no. 2, pp. 284–293, 2020.
[10] A. A. Lubis, M. Yunus, J. Naldi, M. Andry, P. Ginting, F. Safitri, dan M. A. Nasution, “Uji aktivitas antihiperurisemia ekstrak daun kopasanda (Chromolaena odorata (L.) R.M. King & H. Rob) terhadap mencit putih jantan (Mus musculus L.) yang diinduksi kalium oksonat,” Journal of Pharmaceutical and Sciences, pp. 1273–1281, 2023.
