The Comparison Between Total Flavonoid and Antioxidant Activity of Ethyl Acetate Fraction of Moringa Seeds (Moringa oleifera L.) and Winged Bean Seeds (Psophocarpus tetragonolobus L.)

  • Annisa Krisridwany Universitas Muhammadiyah Yogyakarta
  • Miftah Rizkia Tatra
  • Dyani Primasari Sukamdi

Abstract

Moringa seeds (Moringa oleifera L.) and winged bean seeds (Psophocarpus tetragonolobus L.) are medicinal plants used by the community as food and as traditional medicine preparations. They also contain compounds that have potential as antioxidants. It is necessary to compare the levels of one of the compounds and test the antioxidant activity. This study aims to determine the comparison of total flavonoid levels and antioxidant activity in the ethyl acetate fraction of Moringa oleifera L. and winged bean seeds (Psophocarpus tetragonolobus L).

The ethanol extracts were partitioned by using n-hexane, ethanol, and ethyl acetate. This research focused on the ethyl acetate fraction. The total flavonoid determination was based on measuring each fraction's absorbance value at a wavelength of 446 nm and using a quercetin comparator. The antioxidant activity test of ethyl acetate fraction of Moringa oleifera L. and winged bean seeds (Psophocarpus tetragonolobus L.) was carried out by measuring the reduction of each fraction against DPPH radicals (1,1- diphenyl 2-picrilhidrazil) using UV-Vis spectrophotometry method at a wavelength of 517 nm. The antioxidant activity test was carried out by using a comparison of vitamin C.

The results showed the total flavonoid level of ethyl acetate fraction of Moringa oleifera L seeds and winged bean seeds (Psophocarpus tetragonolobus L.) were 42.80 ± 1.61 mg QE/g and 71.56 ± 5.50 mg QE/g, respectively while the antioxidant activities of ethyl acetate fraction of Moringa oleifera L seeds and winged bean seeds (Psophocarpus tetragonolobus L.)  were 134.34 ppm and 179.57 ppm, respectively. The result showed that the ethyl acetate fraction of Moringa seeds (Moringa oleifera L.) had moderate antioxidant activity and more potent compare to Winged bean seeds; however, Winged bean seeds's flavonoid total was higher than Moringa seeds’s.

 

Keyword : Antioxidant; DPPH; Flavonoid; Moringa oleifera L., Psophocarpus tetragonolobus L., seed

Keywords: Antioxidant; Flavonoid; Moringa oleifera L. seed, Psophocarpus tetragonolobus L. seed

References

[1] Reynertson KA. Phytochemical Analysis Of Bioactive Constituents From Edible Myrtaceae Fruits. Dissertation. The City University of New York, 2007.
[2] Mendieta-Araica B, Spörndly E, Reyes-Sánchez N, Salmerón-Miranda F, Halling M. Biomass production and chemical composition of Moringa oleifera under different planting densities and levels of nitrogen fertilization. Agroforest Syst 2013;87:81–92. https://doi.org/10.1007/s10457-012-9525-5.
[3] Fahey J. Microbiological Monitoring of Laboratory Mice. In: Sundberg J, editor. Genetically Engineered Mice Handbook, vol. 20051540, CRC Press; 2005, p. 157–64. https://doi.org/10.1201/9781420039078.ch12.
[4] Jegadeesan GB, Srimathi K, Santosh Srinivas N, Manishkanna S, Vignesh D. Green synthesis of iron oxide nanoparticles using Terminalia bellirica and Moringa oleifera fruit and leaf extracts: Antioxidant, antibacterial and thermoacoustic properties. Biocatalysis and Agricultural Biotechnology 2019;21:101354. https://doi.org/10.1016/j.bcab.2019.101354.
[5] Saleem A, Saleem M, Akhtar MF. Antioxidant, anti-inflammatory and antiarthritic potential of Moringa oleifera Lam: An ethnomedicinal plant of Moringaceae family. South African Journal of Botany 2020;128:246–56. https://doi.org/10.1016/j.sajb.2019.11.023.
[6] Aminah S, Ramdhan T, Yanis M. Kandungan Nutrisi dan Sifat Fungsional Tanaman Kelor (Moringa oleifera). Buletin Pertanian Perkotaan 2015;5:35–44.
[7] Safitri V. Uji Aktivitas Antioksidan Fase n-Hexan, Etil Asetat, dan Air Dari Ekstrak Etanol 96% Daun Kecipir (Psophocarpus tetragonolobus (L.). Skripsi. Universitas Pancasila, 2018.
[8] Adegboyega TT, Abberton MT, AbdelGadir AH, Dianda M, Maziya-Dixon B, Oyatomi OA, et al. Nutrient and Antinutrient Composition of Winged Bean ( Psophocarpus tetragonolobus (L.) DC.) Seeds and Tubers. Journal of Food Quality 2019;2019:1–8. https://doi.org/10.1155/2019/3075208.
[9] Robinson. Kandungan organik tumbuhan tingkat tinggi. Bandung: Institut Teknologi Bandung; 1995.
[10] Dai J, Mumper RJ. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules 2010;15:7313–52. https://doi.org/10.3390/molecules15107313.
[11] McCullough ML, Peterson JJ, Patel R, Jacques PF, Shah R, Dwyer JT. Flavonoid intake and cardiovascular disease mortality in a prospective cohort of US adults. The American Journal of Clinical Nutrition 2012;95:454–64. https://doi.org/10.3945/ajcn.111.016634.
[12] Jahan IA, Hossain MH, Ahmed KS, Sultana Z, Biswas PK, Nada K. Antioxidant activity of Moringa oleifera seed extracts. Orient Pharm Exp Med 2018;18:299–307. https://doi.org/10.1007/s13596-018-0333-y.
[13] Bassal H, Merah O, Ali AM, Hijazi A, El Omar F. Psophocarpus tetragonolobus: An Underused Species with Multiple Potential Uses. Plants 2020;9:1730. https://doi.org/10.3390/plants9121730.
[14] Harborne JB. Metode Fitokimia. Penuntun Cara Modern Menganalisis Tumbuhan. 2nd ed. Bandung: Penerbit ITB; 1987.
[15] Mustikasari K, Ariyani D. The Phytochemistry Screening Of Methanol Extract 2010:6.
[16] Setyowati WAE, Ariani SRD, Rahmawati CP. Skrining Fitokimia Dan Identifikasi Komponen. Seminar Nasional Kimia Dan Pendidikan Kimia VI 2014:10.
[17] Jones WP, Kinghorn AD. Extraction of Plant Secondary Metabolites. Natural Product Isolation. second edition, New Jersey: Humana Press; 2006.
[18] Molyneux P. The use of the stable free radical diphenylpicryl- hydrazyl (DPPH) for estimating antioxidant activity 2004;26:9.
[19] Nihlati IA, Rohman A, Hertiani T. Daya Antioksidan Ekstrak Etanol Rimpang Daya Antioksidan Ekstrak Etanol Rimpang Temu Kunci Temu Kunci [Boesenbergia Pandurata (Roxb.) Schlecth] Dengan Metode Penangkapan Radikal DPPH (1,1-difenil-2-pikrilhidrazil). Universitas Gadjah Mada, 2008.
[20] Tahar N. Penentuan Kadar Flavonoid Dan Kapasitas Antioksidan Ekstrak 2018:10.
[21] Warnasih S, Widiastuti D, Hasanah U, Ambarsari L, Sugita P. Aktivitas Antioksidan Dan Flavonoid Ekstrak Biji Kurma. Ekologia 2020;19:34–8. https://doi.org/10.33751/ekol.v19i1.1660.
[22] Jun M, Fu H-Y, Hong J, Wan X, Yang CS, Ho C-T. Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria lobata Ohwi). J Food Science 2003;68:2117–22. https://doi.org/10.1111/j.1365-2621.2003.tb07029.x.
[23] Marjoni M, Zulfisa A. Antioxidant Activity of Methanol Extract/Fractions of Senggani Leaves (Melastoma candidum D. Don). Pharm Anal Acta 2017;08. https://doi.org/10.4172/2153-2435.1000557.
[24] Unuigbe C, Okeri H, Erharuyi O, Oghenero E, Obamedo D. Phytochemical and antioxidant evaluation of Moringa oleifera (Moringaceae) leaf and seed. J Pharm Bio 2015;11:51. https://doi.org/10.4314/jpb.v11i2.4.
[25] Gu X, Yang Y, Wang Z. Nutritional, phytochemical, antioxidant, α-glucosidase and α-amylase inhibitory properties of Moringa oleifera seeds. South African Journal of Botany 2020;133:151–60. https://doi.org/10.1016/j.sajb.2020.07.021.
Published
2022-04-30