Analysis of Total Flavonoid and Antioxidant Activity of Ethanol Fraction Watermelon Rind (Citrullus lanatus)

  • Feronika Evma Rahayu STIKes Medistra Indonesia
  • Dede Irma Hidayat STIKes Prima Indonesia
  • Tunas Alam STIKes Prima Indonesia

Abstract

Antioxidants are required to keep the body's defenses against free radicals intact. Watermelon rind (Citrullus lanatus (Thunb). & Nakai) contains phenolic compounds which are included in the flavonoid group and can act as natural antioxidant compounds. This study evaluated watermelon rind (Citrullus lanatus (Thunb). & Nakai) for its antioxidant activity. 

The dried watermelon rind (Citrullus lanatus (Thunb). & Nakai) was extracted using the maceration method and then partitioned into polar (water), non-polar (n-hexane), and semi-polar (ethyl acetate) fractions. Following extraction, the study's methodology is briefly outlined in the second paragraph and is in line with the findings of this publication.

The highest total flavonoid value was found in the watermelon rind (Citrullus lanatus (Thunb). & Nakai) ethanol extract, which measured 49.6 mg/g in the ethyl acetate fraction, 48.6 mg/g in the water fraction, and12.05 mg/g in the n-hexane fraction. The ethyl acetate fraction has strong antioxidant activity (55.36), followed by the water fraction (71.25), and the n-hexane is not strong (225.59).

Keywords: watermelon rind; antioxidant activity; total flavonoids

References

[1]. Winarsi H. Antioksidan Alami & Radikal Bebas. Yogyakarta: PT Kanisius. 2007.
[2]. Karadag A, Ozcelik B, Saner S. Review of Methods to Determine Antioxidant Capacities. Food Anal Methods. 2009; 2(1) : 41-60.
[3]. Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In Vitro Antioxidant Activity, Total Phenolic and Flavonoid Contents of Ethanol Extract of Stem and Leaf of Grewia Carpinifolia. Beni-Suef Univ J Basic Appl Sci [Internet]. 2017; 6(1); 10-4.
[4]. Safitri A, Rosdiana A. Biokimia Bahan Alam: Analisis dan Fungsi. Media Nusa Creative: Malang. 2020.
[5]. Lubis W, Intan DR, Harahap WU. Pemanfaatan Limbah Semangka Sebagai Bahan Baku Pembuatan Manisan Dalam Upaya Peningkatan Keterampilan Keterampilan Dan Pendapatan. Jurnal Pengabdian Masyarakat. 2013: Vol: 1(1), Hal: 1-6.
[6]. Yuslianti ER. Pengantar Radikal Bebas dan Antioksidan. Budi Utama: Yogyakarta. 2018.
[7]. Mariani UA, Fasya G, Fauziyah B, Amalia S. Antioxidant Activity Test of Watermelon (Citrullus lanatus) Fruit Extract. Jurnal Akademika Kimia. 2018: 7(2); 96-101.
[8]. Muthmainnah B. Skrining Fitokimia Senyawa Metabolit Sekunder Dari Ekstrak Etanol Buah Delima (Punica Granatum L.) Dengan Metode Uji Warna. Media Farmasi 2017: 13(02); 23–28.
[9]. Purwanto D, Bahri S, Ridhay A. Uji Aktivitas Antioksidan Ekstrak Buah Purnasjiwa (Kopsia arborea Blume.). Jurnal Riset Kimia. 2017. 3(1); 24–32.
[10]. Asra R, Azni NR, Rusdi, Nessa. Uji Aktivitas Antioksidan Ekstrak Etanol Fraksi Heksan, Fraksi Etil Asetat dan Fraksi Air Daun Kapulaga (Elettaria cardamomum (L.) Maton). Journal of Pharmaceutical And Sciences. 2019. 2(1); 30–37.
[11]. Mabruroh EQ, Mursiti S, Kusumo E. Isolasi dan Identifikasi Senyawa Flavonoid dari Daun Murbei (Morus alba Linn). Indonesian Journal of Chemical Science. 2019. 8(1); 16–22.
[12]. Alam T, Ekayanti M, Permana N, Hadissabil Z. Potential Antioxidant Activity Of Extract And Fraction Of Ciplukan (Physalis angulate) On DPPH (1,1-diphenyl-2-picrylhydrazil). Jurnal Farmasi Indonesia. 2022. 19(1); 193-199.
[13]. Adrianta KA, Udayani NNW, Meriyani H. Aktivitas Antioksidan Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme) Dengan Metode Dpph (1,1-Diphenyl-2-Picryhidrazyl). Jurnal Ilmiah Medicamento. 2017. 3(1); 29–33.
[14]. Syahruddin M, Aswad M, Embu YDPA, Khadijah K. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Murbei (Morus Alba L) Asal Kupang, Nusa Tenggara Timur Dengan Metode DPPH (2,2 Diphenil-1- Picrylhydrazyl). Jurnal Penelitian.2019. 8(1) ; 246–252.
[15]. Nur S, Sami FJRW, Awaluddin A, Afsari MIA. Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan. Jurnal Farmasi Galenika. 2019. 05(01) ; 33–42.
Published
2024-11-30