Effect of Glycerin Concentration on Physical Stability Ointment of Snakehead Fish, Golden Sea Cucumber, Kelulut Honey, Green Betel and Clove Oil
Abstract
Snakehead fish (Channa striata), golden sea cucumber (Stichopus hermanii), kelulut honey (Heterotrigona itama), green betel nut (Piper betle) and clove oil (Syzygium aromaticum) have a synergistic effect in wound healing. This combination of ingredients is formulated in an ointment because it has a long contact time on the skin, so it is expected to be effective in healing wounds. The diversity of compounds from each ingredient can affect and cause preparation instability. The addition of glycerin as a humectant is a way to maintain the physical stability of the preparation because it can reduce water evaporation from the preparation. The purpose of this study was to determine the concentration of glycerin which can produce an ointment with good physical
stability.
Variations in the concentration of glycerin used were 5% (F1) and 2.5% (F2), and then the physical properties and stability of the preparations were tested. The method used is the stability test of the intermediate at 30° ± 2° C/ 65% ± 5% RH for 6 months. The measurement parameters include organoleptic test, homogeneity, spreadability, adhesion, protection, and pH.
The results of the physical stability test showed that formula F2 (glycerin 2.5%) was the most stable formula in maintaining spreadability and adhesion from the 0 to 1 month. All formulas were damaged before testing the stability of physical properties in the 3rd month, so this test was not continued in the 3rd and 6th months.
Keywords:
glycerin, humectant, ointment, snakehead fish
References
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[2] R. Tungadi, “Potensi Ikan Gabus (Ophiocephalus striatus) Dalam Mempercepat Penyembuhan Luka,” Jambura Fish Process. J., vol. 1, no. 1, hal. 46–57, 2019, doi: 10.37905/jfpj.v1i1.4505.
[3] D. W. Damaiyanti, “Karakterisasi Esktrak Air Teripang Emas (Stichopus hermanii),” Dent. J. Kedokt. Gigi, vol. 9, no. 1, hal. 74–81, 2015.
[4] R. Sari dan B. SCS, “Characterization And Potency Of Stichopus Hermanni Ethanol Extract On Oral Wound Healing,” ODONTO Dent. J., vol. 7, no. 1, hal. 9–17, 2020, [Daring]. Tersedia pada: https://medium.com/@arifwicaksanaa/pengertian-use-case-a7e576e1b6bf.
[5] W. Zhang, Y. Cai, X. Chen, T. Ji, dan L. Sun, “Optimized Extraction Based On The Terpenoids Of Heterotrigona Itama Propolis And Their Antioxidative And Anti-Inflammatory Activities,” J. Food Biochem., vol. 44, no. 8, hal. 1–12, 2020, doi: 10.1111/jfbc.13296.
[6] E. F. Palumpun, A. A. G. P. Wiraguna, dan W. Pangkahila, “Pemberian Ekstrak Daun Sirih (Piper Betle) Secara Topikal Meningkatkan Ketebalan Epidermis, Jumlah Fibroblas, Dan Jumlah Kolagen Dalam Proses Penyembuhan Luka Pada Tikus Jantan Galur Wistar (Rattus norvegicus),” J. e-Biomedik, vol. 5, no. 1, hal. 1–7, 2017.
[7] N. Wibowo dan N. Comariyati, “Pengaruh Olesan Minyak Cengkeh (Syzygium Aromaticum L) Terhadap Proses Penyembuhan Luka Insisi Pada Hewan Coba Mencit(mus musculus) Strain Balb/ c,” J. Keperawatan Muhammadiyah, vol. 2, no. 1, hal. 1–7, 2017.
[8] M. Andrie dan W. Taurina, “In Vitro Antibacterial Effectiveness Test of Several Herbal Plant Extract in an Strongest Antibacterial Herbal Attempt to Discover the Topical Against Staphylococus aureus dan Pseudomonas aeruginosae,” Eur. J. Biomed. Pharm. Sci., vol. 6, no. 12, hal. 375–379, 2019.
[9] B. Suherman dan D. Isnaeni, “Formulasi Sediaan Salep Ekstrak Daun Kaktus Pakis Giwang (Euphorbia milii Ch.Des Moulins) Kombinasi Basis Modifikasi PEG 4000 dan PEG 400 serta Aktivitas Antibakteri terhadap Staphylococcus epidermis,” J. Herb. Indones., vol. 1, no. 1, hal. 18–32, 2019.
[10] E. Oktami, F. Lestari, dan H. Aprilia, “Studi Literatur Uji Stabilitas Sediaan Farmasi Bahan Alam,” Pros. Farm., vol. 7, no. 1, hal. 72–77, 2021, [Daring]. Tersedia pada: http://dx.doi.org/10.29313/.v7i1.26117.
[11] L. Baumann, S. Saghari, dan E. Weisberg, Cosmetic Dermatology Principles and Practice. London: McGraw-Hill, 2009.
[12] R. T. Sawiji dan N. W. Utariyani, “Optimasi Komposisi PVA Dan Gliserin Pada Masker Gel Peel Off Ekstrak Kulit Buah Naga (Hylocereus lemairei) Secara Simplex Lattice Design,” JIM J. Ilm. Mahaganesha, vol. 1, no. 1, hal. 18–26, 2022.
[13] C. Shintia, S. R. N. Endah, dan A. Nofriyaldi, “Pengaruh Variasi Konsentrasi Hpmc Dan Gliserin Terhadap Sifat Fisik Gel Hand Sanitizer Ekstrak Etanol Daun Pala (Myristica fragrans Houtt.),” Pharmacoscript, vol. 4, no. 1, hal. 58–69, 2021, doi: 10.36423/pharmacoscript.v4i1.603.
[14] M. Andrie dan W. Taurina, “Accelerated Stability Test for Wound Ointment Combination of Snakehead Fish Extract and Golden Sea Cucumber with Variations of Gelling Agent Carbopol 940 and CMC-Na,” Int. J. Pharm. Res., vol. 14, no. 3, hal. 137–146, 2022.
[15] ICH, Stability Testing of new Drug Substances and Products. London: European Medicines Agency, 2006.
[16] G. Manarisip, H. Rotinsulu, dan Fatimawali, “Standardization Of Green Betel Leaf Extracts (Piper betle L.) and Antibacterial Test Against Pseudomonas aeruginosa,” Pharmacon progr Stud Farm, vol. 9, no. 4, hal. 533–541, 2020.
[17] Sari, Amelia, Maulidya, dan Ami, “Formulasi Sediaan Salep Ekstrak Etanol Rimpang Kunyit (Curcuma longa Linn). Poltekkes Kemenkes Aceh, Lampeneurut, Aceh Besar,” SEL, vol. 3, no. 1, hal. 16–23, 2016.
[18] J. B. Soediono, M. Zaini, D. N. Sholeha, dan N. Jannah, “Uji Skrinning Fitokimia Dan Evaluasi Sifat Fisik Sediaan Salep Ekstrak Etanol Daun Kemangi (Ocimum Sanctum (L.)) Dengan Menggunakan Basis Salep Hidrokarbon Dan Basis Salep Serap,” J. Kaji. Ilm. Kesehat. dan Teknol., vol. 1, no. 1, hal. 17–33, 2019.
[19] Sugiyono, Y. Hernani, dan Mufrod, “Formulasi Salep Ekstrak Air Tokek (Gekko gecko L.) Untuk Penyembuhan Luka,” Media Farm. Indones., vol. 8, no. 1, hal. 1093–1106, 2012.
[20] T. Lestari, B. Yunianto, dan A. Winarso, “Evaluasi Mutu Salep Dengan Bahan Aktif Temugiring, Kencur Dan Kunyit,” J. Kebidanan dan Kesehat. Tradis., vol. 2, no. 1, hal. 8–12, 2017, doi: 10.37341/jkkt.v2i1.34.
[21] S. Hastuti, “Pengaruh Pemberian VCO ( VIRGIN COCONUT OIL ) Terhadap Stabilitas Salep Ekstrak Etil asetat Daun Seligi ( Phyllanthus buxifolius Muell . Arg ),” Indones. J. Med. Sci., vol. 4, no. 2, hal. 157–163, 2017.
[22] A. Sari dan A. Maulidya, “Formulasi Sediaan Salep Ekstrak Etanol Rimpang Kunyit (Curcuma longa Linn),” SEL J. Penelit. Kesehat., vol. 3, no. 1, hal. 16–23, 2016.
[23] C. Sussman dan B. Jensen, Wound Care: A Collaborative Practice Manual For Health Proffesionals. Los Angeles: Wolters Kluwer, 2012.
[24] C. Tyastirin dan I. Hidayati, Statistik Parametrik untuk Penelitian Kesehatan. Surabaya: Program Studi Arsitektur UIN Sunan Ampel, 2017.
[25] M. P. Mahardika, “Formulasi dan Evaluasi Sediaan Gel Hand Sanitizer dengan Bahan Aktif Triklosan 0,5%, 0,75% dan 1%,” J. Farm. Dan Kesehat. Indones., vol. 2, no. 1, hal. 1–8, 2022.
[26] N. A. Sayuti, “Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina (Cassia alata L.),” J. Kefarmasian Indones., vol. 5, no. 2, hal. 74–82, 2015.
[27] A. F. Tsabitah, A. K. Zulkarnain, M. S. H. Wahyuningsih, dan D. A. A. Nugrahaningsih, “Optimasi Carbomer, Propilen Glikol, dan Trietanolamin Dalam Formulasi Sediaan Gel Ekstrak Etanol Daun Kembang Bulan (Tithonia diversifolia),” Maj. Farm., vol. 16, no. 2, hal. 111–118, 2020, doi: 10.22146/farmaseutik.v16i2.45666.
[28] Jefri, M. Andrie, dan W. Taurina, “Pengaruh Carbopol 940 Terhadap Stabilitas Fisik Sediaan Salep Kombinasi Ekstrak Ikan Gabus Dan Teripang Emas,” Med. Sains J. Ilm. Kefarmasian, vol. 8, no. 1, hal. 51–64, 2023.
[29] H. D. Azmi, W. A. Subaidah, dan Y. Juliantoni, “Optimasi Formula Sediaan Lotion Ekstrak Daun Jambu Biji (Psidium guajava L.) Dengan Variasi Konsentrasi Setil Alkohol dan Gliserin,” Acta Pharm. Indones., vol. 9, no. 1, hal. 11–20, 2021, doi: 10.20884/1.api.2021.9.1.3408.
[30] N. Nareswari dan A. Kuncoro, “Pembuatan Salep Minyak Atsiri Daun Jeruk Limau (Citrus amblycarpa) Dan Uji Stabilitas Terhadap Tipe Basis Yang Digunakan,” Biofarmasi J. Nat. Prod. Biochem., vol. 14, no. 2, hal. 63–68, 2016, doi: 10.13057/biofar/f140204.
[31] I. Karnia, S. Hamidah, dan G. A. R. Thamrin, “Pengaruh Masa Simpan Madu Kelulut (Trigona SP) Terhadap Kadar Gula Pereduksi dan Keasaman,” J. Sylva
Sci., vol. 2, no. 6, hal. 1094–1099, 2019.
Published
2024-11-30
Section
Articles
