Analgetic Activity Assay of Etanolic Extract Fractions of Gandarusa (Justicia gendarussa Burm. f) Leaves using Tail Flick Method

Abstract

ABSTRACT
Gandarusa (Justicia gendarussa Burm. f) is a potential plant as analgetic activities.
The aim of the present research was to investigate the analgetic activity with tail flick method on
rat models of ethanol extract and the fractions of gandarusa leaf.
Partitioned fractinations isolated from ethanolic extract of gandarusaleaves with nheksana,
etil asetat and water. Dosage extracts and fractions gandarusa leaf, respectively, are
250 mg/kg bw, 500 mg/kg bw, n-hexane fraction of 314 mg/kg bw, the fraction of 41,05 mg/kg
bw and water fractions 144,8 mg/kg bw, the negative control group CMC and aspirin 360 mg/kg
bw. The results showed the n-heksana fraction has optimal analgesic activity with
antinociceptive percentage 94,58% comparable to aspirin.
The preliminary phytochemical analisys showed the presence of active phytochemical
such as flavonoids, alkaloids, saponins, steroids dan tannins and these compound might be
responsible for analgetic mechanism. The possibility analgetic mechanism of the gandarussa its
stimulate lipomodulin protein biosynthesis which inhibit the action of phospolipase enzimatic
that affecting the enzyme activity arachidonic acid but there is also has gastroprotective activity
with increased mucus production and prevents the release of histamine.