Sparation of 1,4-Terpineol and Safrol from Nutmeg Seed Essential Oil (Myristica Fragrans Houtt) And Shigella dysenteriae Bacteria Activity Test

Abstract

Nutmeg were plants that used as material in food, medicine, perfume and cosmetics
industries. Nutmeg essential oil contain 1,4-terpineol and safrol compounds that predected have
antibacterial activity.
Sparation of 1,4-terpineol and safrol compound from nutmeg essential oil was carried out
by fractional distillation method at 95 mmHg and 200- 225C֯. Analysis of nutmeg essential oil and
sparation carried out by GC-MS and antibacterial activity tests with diffusion and dilution methods.
The Analysis results showed that nutmeg seed essential oil contain 5 mayor compounds,
α-pinena, sabinen, 2-β-pinen, terpineol and miristisin, the sparation result contain 40.05%1-4 and
20.34% safrol. The diffusion test showed that 1.25% nutmeg seed essential oil have 12,11mm
inhibition zone that classified as strong inhibition, and the sparation result have 9,66mm inhibitor
zone that classified as moderately inhibition. The dilution test result of nutmeg essential oil and
the separation result have activity for bactericidal has the same at consentration 1.25%.