Diuretic Activity Test n-Hexane Extract of Ceplukan (Physalis angulata L.) Leaves on Wistar Male Rat (Rattus norvegicus)

Abstract

Ceplukan (Physalis angulata L.) empirically have diuretic effect. This study aimed to determined the diuretic effect of n-hexane extract of ceplukan leaves on Wistar male rats. Ceplukan leaves powder were extracted by maceration with n-hexane solvent. Test animal was used 25 Wistar male rats were divided into 5 groups: group I were given CMCNa 0.5% (negative control), group II (positive control) were given furosemide dose 4.32 mg/200 g BW, group III, IV, V were given n-hexane extract suspension dose of 375, 187.5, 93.75 mg/200 g BW respectivelly. The urine volume were measured in hours-6, 12, 18 and 24. Diuretic effect is obtained by calculating the cumulative urine volume and AUC (Area Under the Curve). The data obtained were analyzed by one way ANOVA with a level of 95% significant, then test by LSD (Least Significant Difference).The results showed that n-hexane extract of ceplukan leaves had diuretic effect. The effective dose of n-hexane extract of ceplukan leaves for diuretic effect was dose 375 mg / 200 g BW.