Antihypertensive Activity Test of Matoa Leaves Extract and Fractions in Male Rats Inducted Angiotensin II With Parameters Renin And Angiotensin II Levels
Abstract
ABSTRACT
Matoa leaves (Pometia pinnata) is a plant thought to have hypertensive activity because it contains kuersetin. Research conducted by (Purwidyaningrum, 2017) extracts and fractions of matoa leaves has antihypertensive activity with an effective dose of 150 mg/kg. Other studies conducted by (Elisa, 2019) effects that can reduce blood pressure are extract doses of 300 mg and 30 mg of ethyl acetate fraction induced by angiotensin II can reduce systolic and diastolic blood pressure. This study aims to determine extracts and fractions of matoa leaves in decreasing renin levels and angiotensin II levels. The extraction method was maseration with 96% ethanol solvent and fractionated by liquids method using n-hexane solvent, ethyl acetate water. In this study used 21 male wistar strain rats divided into 7 groups, namely group I (normal), group II as negative control (CMC 1%), group III as positive control (Irbesartan), group IV (matoa leaves extract 60 mg/200g), Group V (fraction n-hexane 2,34 mg/200g), Group VI (ethyl acetate fraction 9,54 mg/200g), Group VII (water fraction 7,98 mg/200g). The results showed ethanol extract and matoa leaves fractions were not significantly difference in meaning with the positive control group different in reducing blood pressure, renin levels and angiotensin II induced by angiotensin II and were significantly from the normal and negative group. The water fraction (7,98 mg/200g) showed a decrease in systolic blood pressure of 9.0% and diastolic 7.1%, the ethyl acetate fraction showed a decrease in renin level 0f 23.6 %. EDM showed a decrease in 17.2% angiotensin II levels. Keyword : Pometia pinnata; renin levels; angiotensin II levels; induced angiotensin IIReferences
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[14]. Minas. (2016). Angiotensin and Mineralocorticoid Receptor Angiotensin Attenuates Cardiac Oxidative Stress in Angiotensin II-Infused Rats. Clinical Experimental Pharmacol Physiol. University of California, Merced, Department of Pharmacology, Kagawa Medical University, Japan. Vol 42(11), 1-23. https://doi.org/10.1111/1440-1681.12473
[15]. Larson AJ, Symons JD, Jalili T. (2010). Quercetin: A treatment for hypertension. Pharmaceuticals; 3(1), 237-250. https://doi.org/ 10.3390/ph30102
[2]. [WHO] World Health Organization. (2012). Managing for Ration
Medicine Use. Geneva
[3]. Kumar et al. (2011). Solunility dissolution rate and enhancement of irbesartan by solid dispersion technique. Journal Of Pharmaceutical and Clinical Research 4(9), 0974-2441. https://doi.org/10.1248/cpb.59.438.
[4]. Dennison-himmelfarb C., Handler J. and Lackland D.T. (2014). EvidenceBased Guideline for the Management of High Blood Pressure in Adults Report From the Panel Members Appointed to the Eighth Joint National Committee (JNC 8), Am Fam Physician 1:90, 503-504
[5]. Trimedona N. Nurdin H. Darwis Dj & Efdi M. (2015). Isolation of triterpenoid from stem bark of Pometia pinnata Forst & Forst. Journal of Chemical and Pharmaceutical Research: Vol 7(11), 225–227
[6]. Kuspradini H, Pasedan WF, Kusuma IW. (2016). Aktivitas antioksidan dan antibakteri ekstrak daun matoa (pometia pinnata). Jurnal Jamu Indonesia. 1(1), 26-34. https://doi.org/10.29244/jji.v1i15
[7]. Lestyo W, Nugraha AS, Himmah UA. (2021). Penentuan aktivitas antioksidan dan antidiabetes ekstrak daun matoa (Pometia pinnata J. R & G Forts) secara in vitro. Jurnal kefarmasian Indonesia 11(2), 132-141. https://doi.org/10.22435/jki.v11i2.3196
[8]. Suedee A, Tewtrakul S, and Panichayupakaranant P. (2013). Anti-IV-1 integrase compound from Pometia pinnata leaves. Pharmaceutical Biology 51(10), 1256-1261. https://doi.org/10.3109/13880209.2013.786098
[9]. Sharmaet et al. (2010). Preclinical Screening Models For Hypertension in Rodents. Journal of Chemical and Pharmaceutical Research: Vol 3 (1), 458-472
[10]. Purwidyaningrum I, Sukandar EY, Fidrianny. (2017). Antihypertensive activity of extract and fractions of matoa (Pometia pinnata J. R & G Forts) Leaves. Asian J Pharm Clin Res,10(3), 323-328. https://doi.org/10.22159/ajpcr.2017.v10i3.16221
[11]. Elisa. N. (2020). Hypertension profile of angiotensin receptor blocker from matoa leaves extract (Pometia pinnata JR Forster & JG. Forster) in angiotensin II induced male rats with blood volume parameter. STRADA Jurnal Ilmiah Kesehatan, 9(2), 1830-1836. https://doi.org/10.30994/sjik.v9i2.595
[12]. Purwidyaningrum I. (2016). Kajian mekanisme kerja antihipertensi daun matoa (Pometia pinnata J. R & G Forts). http://kinerja.lib.itb.ac.id/farmasi/index.php/bibliografi/detail/203
[13]. Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, and Posey LM. (2017). Pharmacotherapy a pathophysiologic approach, 10th ed New York: McGraw Hill, 45-78
[14]. Minas. (2016). Angiotensin and Mineralocorticoid Receptor Angiotensin Attenuates Cardiac Oxidative Stress in Angiotensin II-Infused Rats. Clinical Experimental Pharmacol Physiol. University of California, Merced, Department of Pharmacology, Kagawa Medical University, Japan. Vol 42(11), 1-23. https://doi.org/10.1111/1440-1681.12473
[15]. Larson AJ, Symons JD, Jalili T. (2010). Quercetin: A treatment for hypertension. Pharmaceuticals; 3(1), 237-250. https://doi.org/ 10.3390/ph30102
Published
2023-04-30
Section
Articles
