Hair Tonic Formulation from Ethanol Extract, Ethanol Fraction, and Chloroform-Methanol Fraction of Secang Wood
Abstract
Secang wood (Caesalpinia sappan L.) has been used as an antioxidant in cosmetic products. The bioactive compound contained in secang wood are brazilin, brazilein, 3´-O-methylbrazilin, sappanone, chalcone, and sappankalkon as secondary metabolite components. This study aims to formulating hair tonic from extracts and fractions of secang wood and to determine the quality of hair tonic formulas using ethanol extract (Eet), ethanol fraction (Fen), and chloroform-methanol fraction (Fkm) of secang wood.
The extraction process was carried out using maceration with 96% ethanol as ellent, after that extact partitioned using n-hexane. Fen was then applied using vacuum column chromatography using chloroform, chloroform: methanol (5:1), and methanol as eluent. Eet, Fen, and Fkm of secang wood were then formulated. Hair tonic evaluation includes organoleptic test, determination of pH value, viscosity determination, homogeneity test, and hair growth activity.
The results showed that the three hair tonic formulas produced red-orange color, smelled aromatic in solution form, had a pH of 7, were homogeneous, had a viscosity of 3 cps, and showed activity on hair growth and hair weight of rabbits. FIII gave significantly different results from negative control (p <0.05) on rabbit hair length data on day 30, so ii can be concluded that FIII shows the best activity. All three formulas were qualify according to standard requirements and has activity on hair growth.
Keywords:
formulation; hair growth activity; hair tonic; secang wood (Caesalpinia sappan L.)
References
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[24]. Akib, N.I., Amin, N.A., Malaka, m.H., dan Baka, W.K. 2016. Development and Evaluation of Waru (Hibiscus tiliaceus Linn.) Leaf and Avocado (Persea Americana Mill.) Fruit Extracts for Hair Growth. International Journal of Chemical, Environtmental and Biological Sciences (IJCEBS). 2(4): 138-142p.
[25]. Utami, SM., Djajadisastra, J., Saputri, FC. 2017. Using Hair Growth Activity, Physical Stability, and Safety Tests to Study Hair Tonics Containing Ethanol Extract of Licorice (Glycyrrhiza glabra Linn.). International Journal of Applied Pharmaceutics. 9(1): 46-47p. doi: 10.22159/ijap.2017.v9s1.20_25.
[2]. Umborowati, M.A dan Rahmadewi. 2012. Rambut Rontok Akibat Lingkungan dan Kosmetik. Jurnal Berkala Ilmu Kesehatan Kulit dan Kelamin. 24(1), 37-38p.
[3]. Schrader, K. and Domsch, A. Cosmetology-Theory and Practitice. Volume 2. Augsbrug :Verlag Chemihe Industrie. 2012. 49-58p.
[4]. Batubara, I., Rafi, M., Sadiah, S., Zaim, M.A., Inarianis, dan Mitsunaga, T. 2010. Brazilin Content, Antioxidative and Lipase Inhibitor Effects Of Sappan Wood (Caesalpinia sappan) From Indonesia. J. Chem. and Chem. Eng. 4: 35-50p.
[5]. Lioe, H.N., Adawiyah, D.R. and Anggraeni, R. 2012. Isolation and Characterization of The Major Natural Dyestuff Component of Brazilwood (Caesalpinia sappan L.). Intl. Food Research J. 19(2): 537-532p.
[6]. Dianasari, N. 2009. Uji Aktivitas Antibakteri Ekstrak Etanol Kayu Secang (Caesalpinia sappan L.) Terhadap Staphylococcus aureus dan Shigella dysentriae Serta Bioautografinya. Skripsi. Fakultas Farmasi. Universitas Muhammadiyah Surakarta. Surakarta.
[7]. Noudeh, G.D., Sharififar fariba, Khazaeli Payam., Mohajeri Ehsan, Jahanbakhsh Javad. 2011. Formulation of Herbal Conditioner Shampoo by Using Extract of Fenugreek Seeds and Evaluation of its Physicochemical Parameters. African Journal of Pharmacy and Pharmacology. 5(22): 2420p.
[8]. Rina, Oktaf. 2013. Identifikasi Senyawa Aktif dalam Ekstrak Etanol Kayu Secang (Caesalpinia sappan. L.). Prosiding Semirata FMIPA Universitas Lampung. Lampung.
[9]. Kim, S.H., Lyu, H.N., Kim, S., Jeon, Y.H., Kim, W., Lim, J.K., Lee, H.W., Baek, N.I., Choi, K.Y., Lee, J.T. and Kim, K.T. 2014. Brazilin Isolated From Caesalpinia sappan Suppresses Nuclear Envelope Reassembly By Inhibiting BAF Phosphorylation. ASPET J. DOI: 10.1124/jpet.114.218792
[10]. Padmaningrum, R.T., Marwati, S. dan Wiyarsi, A. 2012. Karakter Ekstrak Zat Warna Kayu Secang (Caesalpinia sappan L.) Sebagai Indikator Titrasi Asam Basa, Prosiding Seminar Nasional Penelitian, Pendidikan dan Penerapan MIPA. Fakultas MIPA. Universitas Negeri Yogyakarta. Yogyakarta.
[11]. Sarker, S.D., Latif, Zahid., Gray, A.I. Natural Products Isolation. Second Edition. New Jersey: Humana Press. 2005.
[12]. Sari, D.Y, Widiyantoro Ari, dan Alimuddin, A.H. 2018. Isolasi Brazilin Dari Kayu Secang (Caesalpinia sappan L.) and Its Formulation For Lipstick. Jurnal Orbital, Jurnal Ilmu dan Terapan Kimia. 1(3): 4-5p.
[13]. Hangoluan, B.Y.M., 2011, Pengembangan Metode Isolasi Brazilin Dari Kayu Secang (Caesalpinia sappan), Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor.
[14]. Santosa, D. dan Haresmita, P.P. 2015. Penentuan Aktivitas Antioksidan Garcinia dulcis (Roxb.) Kurz, Blumeamollis (D.Don) Merr., Siegesbeckia orientalis L., dan Salvia riparia H.B.K yang Dikoleksi Dari Taman Nasional Gunung Merapi Dengan Metode DPPH (2,2-difenil-1-pikril-hidrazil) Serta Profil Kromatografi Lapis Tipisnya. Trad. Med. J., 20(1): 29-30p.
[15]. Desriani., Azizah, Nur., Wahyuni, Ririn., Putri, A.E.P. 2018. Formulasi Hair Tonic Ekstrak Buah Mentimun (Cucumis sativus) sebagai Solusi Ketombe dan Rambut Rontok pada Wanita Berhijab. Pharmauho, 4(1): 39-40p.
[16]. Badan Standar Nasional. Petunjuk Pengujian Organoleptik dan atau Sensori. Standard Nasional Indonesia (SNI). 01-2346-2011. Jakarta: Badan Standard Nasional Indonesia. 2011.
[17]. Badan Standar Nasional. Lotion Hair Tonic. Standard Nasional Indonesia (SNI). 16-4955-1998. Jakarta: Badan Standard Nasional Indonesia. 1998.
[18]. Sona, F.R, 2018. Formulasi Hair Tonic Ekstrak Lidah Buaya (Aloe vera (L.) Burm F.) dan Uji Aktivitas Pertumbuhan Rambut Pada Tikus Putih Jantan. Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan. Universitas Islam NegeriMaulana Malik Ibrahim. Malang.
[19]. Sekar, M., Priya, N.V., Ramesh, R., Kumar G.S. 2016. Formulation and Evaluation of Hair Tonic from Ziziphus jujube Leaf Extract. International Journal of Pharmaceutical, Chemical, and Biological Sciences. 6(4): 448-449p.
[20]. Febriani, A., Elya, B., Jufri, M. 2016. Uji Aktivitas dan Keamanan Hair Tonic Ekstrak Daun Kembang Sepatu (Hibiscus rosa-sinensis) pada Pertumbuhan Rambut Kelinci. Jurnal Farmasi Indonesia. 8(1): 262-264.
[21]. Luliana, S., Desnita, R., Rawinda, R. 2018. Formulation of Hair Grower in Male White Rat (Rattus norvegicus) Wistar Strain. International Journal for Pharmaceutical Research Scholars. 7(3): 138-139.
[22]. Sheskey, P.J., Cook, W.G., Cable, C.G. Handbook of Pharmaceutical Excipient. Eighth Edition. London: Pharmaceutical Press. 2017.
[23]. Amin Juheini, Simamora, E.L.P., Anwar Effionora, Djajadisastra Joshita. 2014. Green Tea (Camelia sinensis L.) Ethanolic Extract as Hair Tonic in Neutraceutical: Physical Stability, Hair Growth Activity on Rats, and Safety Test. International Journal of Pharmacy and Pharmaceutical Sciences. 6(5): 95p.
[24]. Akib, N.I., Amin, N.A., Malaka, m.H., dan Baka, W.K. 2016. Development and Evaluation of Waru (Hibiscus tiliaceus Linn.) Leaf and Avocado (Persea Americana Mill.) Fruit Extracts for Hair Growth. International Journal of Chemical, Environtmental and Biological Sciences (IJCEBS). 2(4): 138-142p.
[25]. Utami, SM., Djajadisastra, J., Saputri, FC. 2017. Using Hair Growth Activity, Physical Stability, and Safety Tests to Study Hair Tonics Containing Ethanol Extract of Licorice (Glycyrrhiza glabra Linn.). International Journal of Applied Pharmaceutics. 9(1): 46-47p. doi: 10.22159/ijap.2017.v9s1.20_25.
Published
2021-11-20
Section
Articles
