The Comparison of Mycobacterium tuberculosis Detection using Molecular Rapid Test and Immunochromatography in Patients Suspected of Having Tuberculosis in Pangkajene and Kepulauan Regencies

  • Faradillah Suhar Biomedical Science Department, Postgraduate Program of Universitas Hasanuddin
  • Muhammad Nasrum Massi Microbiology Department, Faculty of Medicine, Universitas Hasanuddin
  • Rizalinda Sjahril Microbiology Department, Faculty of Medicine, Universitas Hasanuddin

Abstract

Mycobacterium tuberculosis is an acid-resistant bacterium that causes tuberculosis and can be detected using a variety of methods. This study aimed to determine the comparison of the detection results of Mycobacterium tuberculosis using the molecular rapid test (MRT) and immunochromatographic method. This research was conducted at Batara Siang Hospital, Pangkajene, and Kepulauan Regencies, in July-September 2019 using 100 samples. This study is cross-sectional research, applying the chi-square test for analysis. The results of statistical tests revealed a significant difference between the results of tests using molecular rapid tests and immunochromatography (<0.001). The sensitivity, specificity, positive probability value, negative probability value, and accuracy of the immunochromatography method against the molecular rapid test (MRT) were 91.3%, 100%, 100%, 93.1%, and 96%, respectively.

Keywords: rapid molecular test, immunochromatography, tuberculosis

References

Aryati. (2012). One step Mycobacterium tuberculosis antigen rapid test. Dalam: Purwanto A, Hendro P, penyunting. Continuing Professional Development on Laboratory Medicine; Joglosemar IV, 22‒24 Juni 012: Perhimpunan Dokter Spesialis Patologi Klinik Cabang Semarang; 88‒100.
Dinas Kesehatan Kabupaten Pangkep. (20190. Profil Kesehatan Kabupaten Pangkep.
Fransiska, M., & Hartati, E. (2019). Faktor Resiko Kejadian Tuberculosis. Jurnal Kesehatan Institut Kesehatan Prima Nusantara Bukittinggi, 252-260.
Gustiani, N., Parwati, I., Tjandrawaty, A., & Lismayanti, L. (2014). Validitas Pemeriksaan Complex Specific Antigen Mycobacterium tuberculosis Region of Difference 1‒3 Metode Rapid Immunochromatography pada Sputum Penderita Tuberkulosis Paru. MKB, 241-246.
Jeong, Y. J., Lee, K. S., & Yim, J. J. (2017). The Diagnosis of Pulmonary Tuberculosis. Precision and Future Medicine, 77-87.
Kemenkes RI. (2018). Data dan Informasi Profil Kesehatan Indonesia. Jakarta: Kemenkes RI.
Kurniawan, E., Raveinal, Fauzar, & Arsyad, Z. (2016). Nilai diagnostik metode “Real Time” PCR GeneXpert pada TB paru BTA negatif. Jurnal Kesehatan Andalas, 730-738.
Madjawati, Ana. (2010). Uji diagnostik gambaran lesi foto torax pada penderita dengan klinis tuberkulosis paru. Mutiara Medika, 10 (2), 180-188.
Pangaribuan, L., Kristina, Perwitsari, D., Tajayanti, T., & Lolong, D. B. (2020). Faktor-Faktor Risiko yang Mempengaruhi Kejadian Tuberkulosis pada Umur 15 Tahun Ke Atas di Indonesia (Analisis Data Survei Prevalensi Tuberkulosis (SPTB) di Indonesia 2013-2014). Buletin Penelitian Sistem Kesehatan, 10-17.

Shenoy, V.P. & Mukhopadhyay, C. (2014). Rapid Immunochromatograpic Test for The Identification and Discrimination of Mycobacterium tuberculosis Complex Isolates from Non-tuberculous Mycobacteria. Journal of Clinical and Diagnostic Research. 8(4), 13-15.
Shi, J. d. (2018). GeneXpert MTB/RIF Outperforms Mycobacterial Culture in Detecting Mycobacterium tuberculosis from Salivary Sputum. Biomed Research International, 1-5.
Singh, L. & Grover, N. (2011). Detection of TB Antigen by Rapid Test Kit. Med J Armed Forces India. 67(2), 196-197.
Sodiqah, Y., Massi, M., & Sjahril, R. (2018). Efektifitas TBAg (TB Antigen Rapid Test) Pada Isolat Mycobacterium tuberculosis dan Sputum. JST Kesehatan, 308-313.
Van Rie, A. et al. (2013). Point-of-care Xpert MTB/RIF for smear-negative tuberculosis suspects at a primary care clinic in South Africa. Int J Tuberc Lung Dis, 362-372.
WHO. (2019). Global Tuberculosis Report 2019. France: World Health Organization.
Yasmin Asih, N. G., & Effendy, C. (2004). Keperawatan Medikal Bedah : Klien dengan Gangguan Sistem Pernafasan. Jakarta: EGC.
Zeka, A.N., Tasbakan, S., & Cavusoglu, C. (2011). Evaluation of the GeneXpert MTB/RIF Assay for Rapid Diagnosis of Tuberculosis and Detection of Rifampin Resistance in Pulmonary and Extrapulmonary Specimens. Journal of Clinical Microbiology, 49(12), 4138-4141.
Published
2021-09-18
How to Cite
Suhar, F., Massi, M., & Sjahril, R. (2021). The Comparison of Mycobacterium tuberculosis Detection using Molecular Rapid Test and Immunochromatography in Patients Suspected of Having Tuberculosis in Pangkajene and Kepulauan Regencies. Biomedika, 14(2), 62-68. https://doi.org/https://doi.org/10.31001/biomedika.v14i2.981