The Effect of Soursop Fruit Ethanol Extract (Annona Muricata L.) on Low Density Lipoprotein (LDL) Cholesterol Levels White Rat (Rattus Norvegicus) Hipercolesterolemia

  • Siti Fatimah Program Studi D-III Analis Kesehatan, STIKes Guna Bangsa
  • Desto Arisandi Program Studi D-III Analis Kesehatan, STIKes Guna Bangsa
  • Sismawati Sismawati Program Studi D-III Analis Kesehatan, STIKes Guna Bangsa

Abstract

The cause of Coronary heart disease is an increase caused due to increased cholesterol levels in the blood, so there is a  risk of blockage in the blood vessels (atherosclerosis). LDL cholesterol which is abundant in the bloodstream will accumulate in the arteries and potentially cause blockages. Ethanol extract of soursop fruit (Annona Muricata L.) contains antioxidant compounds such as alkaloids, tannins, saponins, flavonoids and polyphenols that are needed to increase endurance and prevent various diseases that enter the body. Research has been conducted to determine the effect of soursop fruit ethanol extract (Annona Muricata L.) on Low Density Lipoprotein (LDL) cholesterol levels white Rat (Rattus Norvegicus) hipercolesterolemia. This research was conducted experimentally with 10 male white rats (Rattus Novergicus, L.), aged 2-3 months with a weight of 150-200 g, which were divided into 2 groups: the control group and the treatment group. Hypercholesterolemia induction is done by giving high fat diet foods for 7 days. Soursop fruit ethanol extract was given in a sonde to the treatment group at a dose of 3.6 mg / 200gBB / day for 14 days and measurements of LDL cholesterol levels were carried out on days 16 and 30 by the CHOD-PAP examination method. Data were analyzed by Paired t-test. The results showed LDL cholesterol levels in the treatment group showed that as many as 5 mice decreased, the mean decreased by 32.49 mg / dl while in the control group as many as 2 mice decreased, the mean decreased by 0.76 mg / dl. There was a significant difference (p <0.05) in changes in LDL cholesterol levels in the treatment group. Soursop fruit etanol extract with 3.6 mg / 200gBB / day for 14 days can reduce LDL cholesterol levels in hypercholesterolemia rats.

Keywords: hypercholesterolemia rats, LDL, soursop fruit ethanol extract

References

Abbas, S.I., Mohammed, T.A., Al-Mahdi RA. 2015. Identificatinon Ofanofof Some Annona murcicata L. (Soursop) Components and Their Antioxidant Effects in rats. The Iraqi Postgraduate Medical Journal, Vol. 14, No.4, 2015.

Anies. 2015. Kolesterol dan Penyakit Jantung Koroner: Solusi Pencegahan dari Aspek Kesehatan Masyarakat. Yogyakarta: Ar-Ruzz Media: 60.

Dharma, S., Supanda, O., Elisma. 2014. Pengaruh Ekstrak Etanol Daun Sirsak (Annona muricata L.) Terhadap Kadar LDL pada Tikus Putih Jantan.Jurnal Farmasi Hige, Vol. 6, No. 1, 2014.

Departemen Kesehatan RI. 2014. Situasi Kesehatan Jantung. Pusat Data dan Informasi, Jakarta Selatan.

Firmansyah D, Bachri MS, Nurkhasanah. 2016. Pengaruh Pemberian Ekstrak Etanol Dan Kloroform Daun Sirsak Terhadap Kolesterol Total Dan Trigliserida Pada Tikus Yang Diinduksi Aloksan, Pharmaciana, Vol. 6, No.1, 47-54, 2016.

Hustomi, I. & Sujayan A. 2011. Gempur Habis Ragam Penyakit dengan Sirsak. Cahaya Yogyakarta: Atma Pustaka:

Kurniadi, H. & Nurrahmani. 2014. Stop Gejala Penyakit Jantung Koroner, Kolesterol Tinggi, Diabetes Melitus, Hipertensi.Yogyakarta: Istana Media: 88-89.

Linder MC. 2010. Biokimia Nutrisi dan Metabolisme. Jakarta: UI Press: 597.

Nilawati S, Krisnatuti D, Mahendra B, Djing OG. 2008. Care Yourse If Cholesterol. Jakarta:Penebar Plus:9.

Posangi I, Posangi J, Wuisan J, 2012 Efek Ekstrak Daun Sirsak (Annona Muricata L.) Pada Kadar Kolesterol Total Tikus Wistar. Jurnal Biomedik. Vol. 4, No. 1.

Prasetya H. 2012. Peluang Jitu Berternak Tikus Putih. Yogyakarta:Baru Press: 77.

Rahman H, Arifin H, Dewi GK, Rizal Z. 2014. Pengaruh Pemberian Buah Sirsak (Annona muricata L.) Terhadap Kadar Asam Urat Darah Mencit Putih Jantan Hiperuresemia. Prosiding Seminar Nasional dan Workshop.Sekolah Tinggi Ilmu Farmasi, Padang.

Risky IS, Kusharyanti I, dan Handini M. 2014. Uji Efek Ekstrak Etanol 70% Daun Bawang Kucai (Allium Tuberosum Rottl. Ex Spreng) Terhadap Penurunan Low Density Lipoprotein (LDL) Serum Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar.Naskah Publikasi, Universitas Tanjung Pura Pontianak.

Rofida S, Firdiansyah A, Fitriyastuti E, 2015 Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Annona squamosa L. Journal of Pharmaceutical Science And Pharmacy Practice. Vol 2, No.1, 2015.

Tia HD, Sistiyono, Hendarta NY. 2014. Pengaruh Berbagai Dosis Jus Buah Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Kolesterol Low Density Lipoprotein (LDL) Serum Tikus Putih (Rattus norvegicus) Dislipidemia. Jurnal Teknologi Laboratorium. Volume 3 Nomor 2 tahun 2014.

Uneputty JP, Yamlean PVY, Kojong NS. 2013. Potensi Infusa Daun Sirsak (Annona Muricata L.) Terhadap Kadar Kolesterol Darah Tikus Putih Jantan (Rattus Novergicus). PHARMACON Jurnal Ilmiah Farmasi – UNSRAT Vol. 2 No. 02, 2013.

Winarsi H. 2009. Antioksidan Alami dan Radikal Bebas. Yogyakarta: Kanisius: 19, 177, 178.

Wurdianing I, Nugraheni SA, Rahfiludin Z, 2014, Efek ekstrak daun sirsak (Annona muricata Linn) terhadap profil lipid tikus putih jantan (Rattus Norvegicus). Jurnal Gizi Indonesia. Vol 3.No.1, 2014.

World Health Organization (WHO). 2001. General Guidelinerfor Methodologies on Reasearch and Evaluation of Traditional Medicine. Genava.

Yuniarti L, Dewi MK, Lantika UA, Bhatara T. 2016.Potensi Ekstrak Air Daun Sirsak Sebagai Penurun Kolesterol dan Pengendali Bobot Badan. Acta Veterinaria Indonesiana. Vol. 4, No. 2: 82-87, Juli 2016.
Published
2019-09-30